Aquatic Invertebrate Monitoring: Introduction to Monitoring

Why monitor at all?
Fresh waters are one of the most basic and important of all natural resources; water is essential to all life on earth.  We monitor water quality and quantity so that we can detect impacts to this vital resource.  Hopefully, by monitoring waters on a regular basis we can detect and cleanup problems before they become major impacts that threaten aquatic and terrestrial life.

Why use bugs?
Aquatic invertebrates, a.k.a. water bugs, live in all types of aquatic habitats including springs, rivers, lakes, reservoirs, ponds, and wetlands, and they generally occur in sufficient variety and abundance that they are easy to collect. The designation "water or aquatic bugs" is somewhat deceiving. The Order Hemiptera are the only "true bugs", however, we often use the term "aquatic bugs" to refer to "aquatic invertebrates".  Aquatic invertebrates are organisms which live part or all of their lives in the water and have no internal skeletal system.  

By knowing what species or groups of bugs live in a water body, biologists are able to evaluate the ecological health and productivity of a system.  To learn about how invertebrate assemblages naturally vary or respond to land use impacts throughout a watershed, click on different stream reaches in the watershed to your right.

Surprising to many is just how many individuals and species of water bugs there are.  There can be thousands to tens of thousands of individual bugs per square meter of streambed and hundreds if not a thousand or more unique species in a healthy river reach.  Each species has specific habitat needs and so each species responds a bit differently to changes in either the chemical,

physical, or biological components of their habitat. Some aquatic insects can complete their life in a few weeks, whereas others may take 2-3 years and non-insect invertebrates, such as some mussels have been found to live for 100 years.  Aquatic insects typically live the majority of their life in the water and then leave the water as adults, whereas non-insect invertebrates, such as amphipods (scuds), gastropods (snails) and bivalves (clams and mussels) spend their entire life in the water.  A few additional traits of aquatic invertebrates that makes them ideal monitoring subjects is that they do not generally move around as much as fish and they are easy to collect.  These qualities enable biologists to detect both recent and more historic impacts as well track the recovery of a disturbed system.  Thus, by knowing who is there and who is not there but probably should be, biologists generally have a good deal about the nature and extent of pollution problems.

Even in healthy rivers, most of the species we find fairly rare, so we need to be very thorough when we sample.  For example, for 4200 stream riffle samples from the western United States, we found that only 4 genera occurred in more than 50% of the samples.  The 10 most common genera we have found in the western United States are Baetis, Optioservus, Simulium, Rhyacophila, Cinygmula, Epeorus, Zaitzevia, Hydropsyche, Drunella, and Paraleptophlebia.  Click on the image to the right to make readable! Most of the most common taxa we collect are habitat generalists, that is, they live just about everywhere, so their presence does not tell us much about the health of a river. It is the rarer taxa that are either particularly sensitive to pollution or extremely tolerant of pollution that provide biologists with the most telling facts about a river's health.

Aquatic invertebrate classification schemes
Aquatic invertebrates can be classified or separated according to their life history traits with respect to preferences or life history characteristics. A biref general classification is below.

A detailed classification of aquatic bug traits was recently published:

Nicole K. M. Vieira, N. LeRoy Poff, Daren M. Carlisle, Stephen R. Moulton II, Marci L. Koski, and Boris C. Kondratieff. 2005. A Database of Lotic Invertebrate Traits for North America. U.S. Geological Survey Data Series 178

It can be downloaded here: http://pubs.usgs.gov/ds/ds187/

Excel data files from this work can be downloaded here: Bug trait definitions Bug trait attributes file

 

Classification attribute Common invertebrates
Temperature preferences
Warm stenothermic: waters generally always warm.  May include warm springs.  Coleoptera: Dytiscidae, Hydrophilidae
Diptera: Ephydridae, Stratiomyidae
Hemipterans: Saldidae, Corixidae
Odonata: Coenagrionidae, Libellulidae
Cold stenothermic: waters generally always cold. May include cold springs and high elevation streams and ponds.  Coleoptera: Dytiscidae
Diptera: Chironomidae
Ephemeroptera: Baetidae, Ephemerellidae
Plecoptera: Leuctridae, Nemouridae, Perlidae
Trichoptera: Lepostomatidae, Limnephilidae, Rhyacophilidae
Eurythermal: waters that vary from cold to warm throughout the year.  The majority of streams and ponds fit this category.  Many 
Sediment preferences
Coarse substrates, like gravel and cobbles Diptera: Blephariceridae, Chironomidae, Deuterophlebiidae, Simuliidae 
Ephemeroptera: Ephemerellidae, Heptageniidae
Plecoptera: Chloroperlidae, Perlidae, Perlodidae
Trichoptera: Glossosomatidae, Hydropsychidae, Philopotamidae, Rhyacophilidae
Coarse substrates with abundant leaf litter Coleoptera: Elmidae, Psephenidae
Diptera: Tipulidae
Megaloptera: Corydalidae, Sialidae
Plecoptera: Leuctridae, Nemouridae, Peltoperlidae, Pteronarcyidae
Trichoptera: Brachycentridae, Limnephilidae
Fine substrates, like sands and silts Oligochaeta
Coleoptera: Notonectidae 
Diptera: Chironomidae
Ephemeroptera: Caenidae, Ephemeridae, Tricorythidae
Odonata: Gomphidae
Trichoptera: Lepidostomatidae
Erosional or depositional substrates with abundant aquatic vegetation Coleoptera: Dytiscidae, Gyrinidae, Haliplidae, 
Hemiptera: Belostomatidae, Corixidae
Odonata: Coenagrionidae, Libellulidae
Trichoptera: Leptoceridae
Riparian conditions
Shaded streams: streams shaded by dense riparian vegetation provide lots of food in the form of leaves and wood (also referred to as allochthonous material) that can be used by invertebrates known as shredders. Coleoptera: Elmidae
Plecoptera: Peltoperlidae, Pteronaricyidae
Trichoptera: Calcamoceratidae, Lepodostomidae, Limnephilidae
Open canopy streams: streams without heavy shading generally have more instream primary production than shaded streams and are dominated by bugs that prefer to eat algae or filter fine organic matter (also referred to as autochthonous matter) from the water column. Ephemeroptera: Baetidae
Trichoptera: Hydropsychidae
Nutrient tolerance
Oligotrophic:  waters characterized by low nutrient concentrations. Diptera: Blephariceridae
Ephemeroptera: Ephemerellidae, Heptageniidae
Plecoptera: Chloroperlidae, Nemouridae, Peltoperlidae,  Perlidae, Perlodidae, Pteronarcyidae
Trichoptera: Rhyacophilidae, Glossosomatidae
Eutrophic:  waters characterized by higher nutrient concentrations.  Diptera: Muscidae, Simuliidae, Syrphidae 
Ephemeroptera: Baetidae, Tricorythidae
Trichoptera: Hydropsychidae, Brachycentridae, 
Feeding preferences
Shredders - Invertebrates that eat leaves, twigs and the bacteria and fungi that grow on this woody debris. Diptera: Tipulidae
Ephemeroptera: Ephemerellidae 
Plecoptera: Capniidae, Leuctridae, Nemouridae, Peltoperlidae, Pteronarcyidae
Trichoptera: Lepidostomatidae, Limnephilidae, Sericostomatidae
Scrapers - Invertebrates that scrape or "graze" attached algae and detritus from the surface of rocks, twigs, and other objects.  Coleoptera: Psephenidae 
Diptera: Blephariceridae
Ephemeroptera: Heptageniidae
Plecoptera: Perlodidae, Chloroperlidae, Trichoptera: Calamoceratidae, Glossosomatidae, Leptoceridae, Uenoidae
Collector-gatherers - Invertebrates that feed primarily on algae, detritus, and bacteria deposited on sediments in slow water areas. Ephemeroptera: Baetidae, Caenidae, Ephemerellidae, Ephemeridae, Tricorythidae
Plecoptera: Nemouridae, 
Trichoptera: Brachycentridae, Leptoceridae
Diptera: Chironomidae, Psychodidae, Empididae
Coleoptera: Elmidae
Filter feeders - Invertebrates that filter bacteria, algae, detritus, and animal matter from the water column.  They do this by either constructing nets, or using specialized appendages on their bodies to trap the food as it moves with the water. Trichoptera: Philopotamidae, Polycentropodidae, Hydropsychidae
Diptera: Simuliidae
Predators - Invertebrates that feed primarily on other invertebrates. Coleoptera:   Dytiscidae
Diptera: Ceratopogonidae, Dolichopodidae, Muscidae, Tabanidae 
Hemiptera: all 
Megaloptera: all 
Odonata: Anisoptera, Zygoptera 
Plecoptera: Chloroperlidae, Perlidae, Perlodidae
Trichoptera: Rhyacophilidae
Life history strategies
Holometabola or complete metamorphosis - life cycle includes egg, larvae, pupae, and adult forms or stages. Diptera
Trichoptera
Coleoptera
Heterometabola or incomplete metamorphosis - life cycle includes egg, larvae, and adult forms or stages.  There is no pupal phase. Ephemeroptera
Plecoptera
Odonata
Ametabola or no metamorphosis - life cycle includes egg, immature adults, and adult forms or stages.  Amphipoda
Collembola
Nematoda
Oligochaeta
Life cycle length
Multi-voltine - Life cycle is completed within a few months.  These species have multiple generations within a single year. Diptera: Chironomidae
Ephemeroptera: Baetidae
Uni-voltine - Life cycle is completed in about 1 year, so that these species have only 1 generation per year. Most aquatic insects
Semi-voltine - Long-lived species.  Life cycles take more than a year to complete. Megaloptera: Corydalidae, Sialidae
Odonata: Coenagrionidae
Plecoptera: Perlidae