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Family Genus
Virus
Strain
Adenoviridae Mastadenovirus

Adenovirus

15 serotypes
Arenaviridae Arenavirus

Lymphocytic choriomeningitis

Armstrong E-350

Junin

Candid #1

Tacaribe

TRVL 11573

Pichinde

An 4763
Bunyavirdae Hantavirus

Dobrava

Sotkamo

Sin Nombre

SN77734

Andes

Chile-9717869

Maporal

HV 97021050
Orthobunyavirus

La Crosse encephalitis

Phlebovirus

Punta Toro

Adames

Rift Valley fever

MP-12, ZH501

Sandfly fever

Naples
Coronaviridae Coronavirus

SARS Corona

Urbani, Toronto-2, Frankfurt-1, CuHK-1

Corona

Human, rat, mouse

Transmissible gastroenteritis

Bovine corona

Flaviviridae Flavivirus

Banzi

H336

Dengue

Serotypes 2 and 3

West Nile

NY WNV and WN02

Yellow Fever

17D
Herpesviridae Simplexvirus

Herpes

Types 1 and 2
Varicellovirus

Bovine herpes

Orthomyxoviridae  

Influenza

30 serotypes pandemic and seasonal H1N1, seasonal H3N2, low-path avian H5N1, high-path avian H5N1, and some oseltamivir-resistant and amantadine-resistant strains

Paramyxoviridae
Morbillivirus

Measles

Respirovirus

Parainfluenza

Pneumovirus

Respiratory syncytial

Human A and B strains, clinical isolates and bovine
Picornaviridae Enterovirus

Coxsackie B1

Coxsackie B4

Enterovirus-71

Poliovirus Type 3

Cardiovirus

Encephalomyocarditis

ECHO 12

Rhinovirus

Rhino

100 strains and clinical isolates
Poxviridae Orthopoxvirus

Cowpox

Brighton

Vaccinia

WR and IHD
Reoviridae Reovirus

Reo

Rota

Human, simian, and bovine
Rhabdoviridae Vesiculovirus

Vesicular stomatitis

Togaviridae Alphavirus

Chikungunya

Tanganyika (VR-64)

Semliki Forest

Original

Venezuelan equine encephalitis

TC-83

Western equine encephalitis

California (VR-70)
 

 

Antiviral Assay by CPE:  

This test is for initial screening of potentially antiviral compounds.  The antiviral activity of the compound is evaluated based on the ability of the compound to prevent virus from causing viral cytopathic effects (CPE) in mammalian cell culture.  Eight dilutions of test compound are assayed, and the effective antiviral concentration determined by regression analysis.  The toxicity of the test compound is determined in parallel. An abbreviated test with only 4 dilutions of compound may be employed to screen large numbers of compounds quickly and at a reduced cost.

Virus Yield Reduction Assay:

This test evaluates the ability of the compound to inhibit virus production in mammalian cell culture. This is a two-step assay where virus is first produced in cultures containing the antiviral substance at varying dilutions, followed later by titration of the samples for virus titer by endpoint dilution in 96-well microplates. Eight dilutions of test compound are assayed, and the effective antiviral concentration determined by regression analysis. This test is normally requested as a follow-up for compounds that demonstrate activity in the CPE assay.


Virucidal Assay:

This assay shows whether a test compound inactivates ("kills") virus outside of cells, or in other words, whether the compound inactivates the virus before infection of the cells. A virucidal compound will appear active in the antiviral assay, but virucidal compounds (e.g. alcohol, sodium hypochlorite, etc.) are normally not considered candidates for antiviral treatment.  The assay is performed by incubating virus plus compound for 1 hour (or a length of time requested by the sponsor), followed by determining virus titer by endpoint dilution in 96-well microplates of cells.